Application

Research CategorySignaling

Immunohistological staining of tissue sections on frozen or formalin fixed paraffin wax embedded sections

Optimal working dilutions must be determined by the end user.

Research Sub CategoryInsulin/Energy Signaling

This Anti-Insulin-like Growth Factor-I Antibody, clone M23 is validated for use in IH(P) for the detection of Insulin-like Growth Factor-I.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified human IGF-1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

The monoclonal is presented at a concentration of 100µg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Format: Purified

Specificity

The basic IGF-1-like forms (pl>9.0) showed substantial cross-reactivity, as did rat and mouse sera. Monoclonal antibody BM23 was capable of abolishing the biological activities of IGF-1 in vitro bioassays for insulin-like, mitogenic and sulphation activities.

AFFINITY CONSTANT: The affinity of monoclonal antibody BM23 for IGF-1 was 1 x 10E10 L/mole and the cross reactivities of IGF-2, MSA and porcine insulin were 1%, 0.05% and 0.0001% respectively

Storage and Stability

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.